Bioproducts
By making full use of our technologies having developed over many years for fermentation, extraction, purification and others, we have been manufacturing the enzyme products for clinical diagnostic and others as one of bioproducts materials. We are also promoting joint research with customers on enzyme modification (substrate specificity, etc.) and microbial fermentation.
Enzymes
Enzyme list *
| Substrate | Products | EC Number | Reaction | Applications |
|---|---|---|---|---|
| Saccharide | Glucose dehydrogenase | 1.1.5.9 | D-Glucose + acceptor -> D-Glucono-1,5-lactone + reduced acceptor | ・Quantification of D-glucose ・Blood glucose measurement |
| Pyranose oxidase | 1.1.3.10 | D-Glucose + O2 -> 2-Dehydro-D-glucose + H2O2 | ・Quantification of D-glucose ・Hydrogen peroxide–mediated sterilization ・Synthesis of 2-ketoglucose (D-glucosone) ・1,5-AG measurement |
|
| Glucose 6-dehydrogenase |
- | D-Glucose + acceptor -> D-Glucuronic acid + reduced acceptor | ・Quantification of D-glucose ・Blood glucose measurement ・1,5-AG measurement ・Synthesis of uronic acids |
|
| Amino acid (Oxidase) |
D-Amino acid oxidase | 1.4.3.3 | D-Amino acid + H2O + O2 -> 2-Oxo acid + NH3 + H2O2 | Quantification of D-amino acids |
| L-Amino acid oxidase | 1.4.3.2 | L-Amino acid + H2O + O2 ->
2-Oxo acid + NH3 + H2O2 (L-Phenylalanine + H2O + O2 -> Phenylpyruvic acid + NH3 + H2O2) |
Quantification of L-phenylalanine | |
| Glycine oxidase | 1.4.3.19 | Glycine + H2O + O2 -> Glyoxylic acid + NH3 + H2O2 | Quantification of glycine | |
| L-Kynurenine 3-monooxygenase |
1.14.13.9 | L-Kynurenine + NAD(P)H + H+ + O2 -> 3-Hydroxy-L-kynurenine + NAD(P)+ + H2O |
Quantification of L-kynurenine | |
| L-Tryptophan oxidase | - | L-Tryptophan + H2O + O2 -> Indole-3-pyruvic acid + NH3 + H2O2 | Quantification of L-tryptophan | |
| L-Glutamic acid oxidase | 1.4.3.11 | L-Glutamic acid + H2O + O2 -> 2-Oxo acid + NH3 + H2O2 | Quantification of L-glutamic acid | |
| Amino acid (Others) |
β-Cyanoalanine synthase | 2.5.1.47 | O-Acetyl-L-serine + HCN -> β-Cyano-L-alanine + CH3COOH | Synthesis of β-cyanoalanine (Intermediate in amino acid derivative synthesis) |
| γ-Cyano-α- aminobutyric acid synthase |
2.5.1.49 | O-Acetyl-L-homoserine + HCN-> γ-Cyano-α-aminobutyric acid + CH3COOH | Synthesis of γ-cyanoaminobutyric acid (Intermediate in amino acid derivative synthesis) |
|
| Tryptophanase | 4.1.99.1 | L-Tryptophan + H2O <-> Indole + Pyruvic acid + NH3 | Synthesis of labeled amino acid (L-Tryptophan) | |
| β-Tyrosinase | 4.1.99.2 | L-Tyrosine + H2O <-> Phenol + Pyruvic acid + NH3 | Synthesis of labeled amino acid (L-DOPA) | |
| Aminoacyl-tRNA synthetase |
6.1.1.- | Amino acid＋ATP -> Aminoacyl-AMP＋Diphosphate Aminoacyl-AMP ＋ tRNA -> Aminoacyl-tRNA + AMP |
Quantification of L-amino acid | |
| GABase(γ-Aminobutyric acid aminotransferase(GABA-AT),Succinate semialdehyde dehydrogenase(SSDH)) | 2.6.1.19(GABA-AT) 1.2.1.24(SSDH) |
γ-Amino-n-butyrate + α-Ketoglutarate -> Succinate semialdehyde + Glutamate Succinate semialdehyde + NADP+ -> Succinate + NADPH |
Quantification of γ-aminobutyric acid (GABA) | |
| Glycated amino acid |
Fructosamine 6-kinase | - | ε-Fructoselysine + ATP -> Fructoselysine 6-phosphate + ADP | Glycated albumin measurement |
| Carboxylic acid | Formate dehydrogenase | 1.17.1.9 | Formate + NAD+ <-> CO2 + NADH | ・Quantification of formic acid ・NADH regeneration |
* Some of these products are under development.
Main products
Glucose dehydrogenase
(FAD-GDH)
Glucose dehydrogenase dependent on FAD (flavin adenine dinucleotide) can be used to determine blood glucose. A main feature of this product is that it is not influenced by the presence of maltose, galactose, or oxygen in the blood.
We also deal with enzymes that do not react with xylose.
Pyranose oxidase
Pyranose oxidase can be used to measure pyranose (glucose, 1,5-AG) levels in biological and food samples. A main feature of this product is that it is highly stable in the liquid state and is not inhibited by high salt concentrations.
Amino acid oxidase
We have been developing various amino acid oxidases or oxygenase with high substrate specificity (e.g. D-amino acid, L-Tryptophan, L-Phenylalanine, Glycine and L-Kynurenine) and thermostability. These enzymes can be used for measurement of the substrate levels in samples and for production of 2-oxo acid or hydroxy derivatives.
Manufacturing services and joint development
Manufacturing services
We offer contract manufacturing services regarding such fermentation, extraction and purification.
Click here for details of our bioproducts facilities.
Joint development
We are also promoting joint research with customers on enzyme modification (substrate specificity, etc.) , enzyme manufacturing and microbial fermentation.